Bound protein was washed with Buffer B (20 mM Hepes, pH 7.4, 1M NaCl, 25 mM Imidazole, 10% (vol/vol) glycerol, 6M urea, 6 mM βME) and eluted in Buffer C (20 mM Hepes, pH 7.4, 1M NaCl, 250 mM Imidazole, 10% (vol/vol) Glycerol, 6M Urea, 6 mM…
It isaddressed to Porsche 911 owners and enthusiasts who will enjoy it in their free time and useit as a future reference to their car’s needs. They're lossless and they support the full 00:26:06.16 bit depth of your system. Almost always those custom formats 00:26:10.28 will support multidimensional images, so you can save that 00:26:14.17 entire three channel by 15 z-slice by 200… Blastomere volumes (Figure 1E) were determined using the strain OD58 (Audhya, Hyndman et al. 2005), in which GFP localizes to the plasma membrane. 47 Z slices (1 μm step sizes) covering the full embryo volume were collected with 50% 488 nm… PATag-RFP - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Deepwater Meeting Demand - Free download as PDF File (.pdf), Text File (.txt) or view presentation slides online. Shell deepwater demand For these studies, 293T cells expressing FLAG-tagged DLK were cultured in Silac media containing isotopically enriched (heavy) versions of lysine (13C615N2) and arginine (13C615N4) or their unlabeled counterparts (light). To test whether SPR2 function is required for cortical array reorientation in response to blue light, we measured the rate of cortical array reorientation in etiolated hypocotyl cells of the spr2-2 mutant expressing mCherry-TUA5 to…
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This result suggested Ii-FRB is an inefficient ER trapper for FKBP12-tagged proteins. We then reversed the configuration of the FRB and FKBP probes on EGFP-GPI and Ii, coexpressing Ii-FKBP12-mRFP and FRB-EGFP-GPI.
RAB-5 controls EFF-1 localization to the plasma membrane. a, Schematic of the PLM neuron in the tail of the animal; dashed box indicates the region depicted in b. b, Representative maximum projection confocal images of the PLM neuron in eff… Ch5_Flow - Free download as PDF File (.pdf), Text File (.txt) or read online for free. ce It isaddressed to Porsche 911 owners and enthusiasts who will enjoy it in their free time and useit as a future reference to their car’s needs. They're lossless and they support the full 00:26:06.16 bit depth of your system. Almost always those custom formats 00:26:10.28 will support multidimensional images, so you can save that 00:26:14.17 entire three channel by 15 z-slice by 200… Blastomere volumes (Figure 1E) were determined using the strain OD58 (Audhya, Hyndman et al. 2005), in which GFP localizes to the plasma membrane. 47 Z slices (1 μm step sizes) covering the full embryo volume were collected with 50% 488 nm…